■ 氏名
坂本 修士 (サカモト シュウジ)
SAKAMOTO Shuji

■ 所属部局/職名
Research and Education Faculty, Medical Sciences Cluster, Basic Medicine Unit/Associate Professor
教育研究部 医療学系 基礎医学部門/准教授

■ 学部・学科・コース等
Science Research Center, Life Sciences and Functional Materials Section, The Facility for Research Instruments
総合研究センター 生命・機能物質部門 実験実習機器施設

■ 連絡先
住 所
電 話
F A X
E-Mailsshuji●kochi-u.ac.jp    (送信の際は“●”を半角の“@”に変更して送信してください。)
U R Lhttp://www.kochi-ms.ac.jp/~ct_mrc/academic/

■ 出身大学院・研究科等

■ 出身学校・専攻等

■ 取得学位
医学 () 高知医科大 (現 高知大学 医学部)

■ 研究職歴

■ 委員歴・役員歴

■ 受賞学術賞

■ 所属学会

■ 専門分野
分子生物学

■ 主な研究テーマ・活動
細胞腫瘍化や筋分化・成熟に関与する非翻訳RNAの産生調節に関する研究

■ 研究についてのPR
細胞の腫瘍化や骨格筋の分化・成熟に関与する非翻訳RNAの役割を明らかにしていきたい。

■ 社会人・生徒を対象として可能な講演・授業(例えば講演題目)
細胞分子生物学、セントラルドグマ(DNA → RNA → タンパク質の流れ)に関連する講義

■ 研究業績(論文・解説)
Candidate plasticity gene 16 mediates suppression of insulin gene expression in rat insulinoma INS-1?cells under glucotoxic conditions.
 Biochemical and biophysical research communications (Nakane T, Ido A, Higuchi T, Todaka H, Morisawa K, Nagamine T, Fukunaga K, Sakamoto S, Murao K, Sugiyama Y) /, 201903 () 10.1016/j.bbrc.2019.03.036

A negative feedback loop between nuclear factor 90 (NF90) and an anti-oncogenic microRNA, miR-7.
 Biochemical and biophysical research communications (Higuchi T, Morisawa K, Todaka H, Lai S, Chi E, Matsukawa K, Sugiyama Y, Sakamoto S) 503/3, 1819-1824 201807 () 10.1016/j.bbrc.2018.07.119

An NF90/NF110-mediated feedback amplification loop regulates dicer expression and controls ovarian carcinoma progression.
 Cell research (Barbier J, Chen X, Sanchez G, Cai M, Helsmoortel M, Higuchi T, Giraud P, Contreras X, Yuan G, Feng Z, Nait-Saidi R, Deas O, Bluy L, Judde JG, Rouquier S, Ritchie W, Sakamoto S, Xie D, Kiernan R) 28/5, 556-571 201805 () 10.1038/s41422-018-0016-8

A method for profiling the phosphorylation state of tyrosine protein kinases.
 Biochimica et biophysica acta (Uezato Y, Kameshita I, Morisawa K, Sakamoto S, Kinoshita E, Kinoshita-Kikuta E, Koike T, Sugiyama Y) /, 201805 () 10.1016/j.bbapap.2018.05.003

Suppression of MicroRNA-7 (miR-7) Biogenesis by Nuclear Factor 90-Nuclear Factor 45 Complex (NF90-NF45) Controls Cell Proliferation in Hepatocellular Carcinoma.
 The Journal of biological chemistry (Higuchi T, Todaka H, Sugiyama Y, Ono M, Tamaki N, Hatano E, Takezaki Y, Hanazaki K, Miwa T, Lai S, Morisawa K, Tsuda M, Taniguchi T, Sakamoto S) 291/40, 21074-21084 201609 () 10.1074/jbc.M116.748210

Patatin-like phospholipase domain-containing protein 3 is involved in hepatic fatty acid and triglyceride metabolism through X-box binding protein 1 and modulation of endoplasmic reticulum stress in mice.
 Hepatology research : the official journal of the Japan Society of Hepatology (Ochi T, Munekage K, Ono M, Higuchi T, Tsuda M, Hayashi Y, Okamoto N, Toda K, Sakamoto S, Oben JA, Saibara T) 46/6, 584-592 201605 () 10.1111/hepr.12587

Overexpression of NF90-NF45 Represses Myogenic MicroRNA Biogenesis, Resulting in Development of Skeletal Muscle Atrophy and Centronuclear Muscle Fibers.
 Molecular and cellular biology (Todaka H, Higuchi T, Yagyu K, Sugiyama Y, Yamaguchi F, Morisawa K, Ono M, Fukushima A, Tsuda M, Taniguchi T, Sakamoto S) 35/13, 2295-2308 201507 () 10.1128/MCB.01297-14

Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE.
 MethodsX (Sugiyama Y, Katayama S, Kameshita I, Morisawa K, Higuchi T, Todaka H, Kinoshita E, Kinoshita-Kikuta E, Koike T, Taniguchi T, Sakamoto S) 2/, 469-474 2015 () 10.1016/j.mex.2015.11.007

Bofutsushosan, a Japanese herbal (Kampo) medicine, attenuates progression of nonalcoholic steatohepatitis in mice.
 Journal of gastroenterology (Ono M, Ogasawara M, Hirose A, Mogami S, Ootake N, Aritake K, Higuchi T, Okamoto N, Sakamoto S, Yamamoto M, Urade Y, Saibara T, Oben JA) 49/6, 1065-1073 201406 () 10.1007/s00535-013-0852-8

E2A and CBP/p300 act in synergy to promote chromatin accessibility of the immunoglobulin κ locus.
 Journal of immunology (Baltimore, Md. : 1950) (Sakamoto S, Wakae K, Anzai Y, Murai K, Tamaki N, Miyazaki M, Miyazaki K, Romanow WJ, Ikawa T, Kitamura D, Yanagihara I, Minato N, Murre C, Agata Y) 188/11, 5547-5560 201206 () 10.4049/jimmunol.1002346

Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line.
 American journal of cancer research (Takaoka E, Sonobe H, Akimaru K, Sakamoto S, Shuin T, Daibata M, Taguchi T, Tominaga A) 2/2, 141-152 2012 ()

High expression of nuclear factor 90 (NF90) leads to mitochondrial degradation in skeletal and cardiac muscles.
 PloS one (Higuchi T, Sakamoto S, Kakinuma Y, Kai S, Yagyu K, Todaka H, Chi E, Okada S, Ujihara T, Morisawa K, Ono M, Sugiyama Y, Ishida W, Fukushima A, Tsuda M, Agata Y, Taniguchi T) 7/8, e43340 2012 () 10.1371/journal.pone.0043340

Regulation of experimental autoimmune uveoretinitis by anti-delta-like ligand 4 monoclonal antibody.
 Investigative ophthalmology & visual science (Ishida W, Fukuda K, Sakamoto S, Koyama N, Koyanagi A, Yagita H, Fukushima A) 52/11, 8224-8230 201110 () 10.1167/iovs.11-7756

Regulation of TCR Vγ2 gene rearrangement by the helix-loop-helix protein, E2A.
 International immunology (Nozaki M, Wakae K, Tamaki N, Sakamoto S, Ohnishi K, Uejima T, Minato N, Yanagihara I, Agata Y) 23/5, 297-305 201105 () 10.1093/intimm/dxr005

A novel and comprehensive mouse model of human non-alcoholic steatohepatitis with the full range of dysmetabolic and histological abnormalities induced by gold thioglucose and a high-fat diet.
 Liver international : official journal of the International Association for the Study of the Liver (Ogasawara M, Hirose A, Ono M, Aritake K, Nozaki Y, Takahashi M, Okamoto N, Sakamoto S, Iwasaki S, Asanuma T, Taniguchi T, Urade Y, Onishi S, Saibara T, Oben JA) 31/4, 542-551 201104 () 10.1111/j.1478-3231.2010.02443.x

Dynamic changes of microRNAs in the eye during the development of experimental autoimmune uveoretinitis.
 Investigative ophthalmology & visual science (Ishida W, Fukuda K, Higuchi T, Kajisako M, Sakamoto S, Fukushima A) 52/1, 611-617 201102 () 10.1167/iovs.10-6115

The NF90-NF45 complex functions as a negative regulator in the microRNA processing pathway.
 Molecular and cellular biology (Sakamoto S, Aoki K, Higuchi T, Todaka H, Morisawa K, Tamaki N, Hatano E, Fukushima A, Taniguchi T, Agata Y) 29/13, 3754-3769 200907 () 10.1128/MCB.01836-08

Systemic overexpression of IFN-gamma and IL-5 exacerbates early phase reaction and conjunctival eosinophilia, respectively, in experimental allergic conjunctivitis
 Br. J. Ophthalmol (Ishida W, Tsuru E, Tominaga A, Miyazaki J, Higuchi T, Sakamoto S, Fukushima A) 93/12, 1680-1685 2009 () 10.1136/bjo.2009.162594

Regulation of T cell receptor beta gene rearrangements and allelic exclusion by the helix-loop-helix protein, E47.
 Immunity (Agata Y, Tamaki N, Sakamoto S, Ikawa T, Masuda K, Kawamoto H, Murre C) 27/6, 871-884 200712 () 10.1016/j.immuni.2007.11.015

Type I interferons activate apoptosis in a Jurkat cell variant by caspase-dependent and independent mechanisms.
 Cellular signalling (Gamero AM, Potla R, Sakamoto S, Baker DP, Abraham R, Larner AC) 18/8, 1299-1308 200608 () 10.1016/j.cellsig.2005.10.008

Novel roles of TLR3 tyrosine phosphorylation and PI3 kinase in double-stranded RNA signaling.
 Nature structural & molecular biology (Sarkar SN, Peters KL, Elco CP, Sakamoto S, Pal S, Sen GC) 11/11, 1060-1067 200411 () 10.1038/nsmb847

Histone deacetylase activity is required to recruit RNA polymerase II to the promoters of selected interferon-stimulated early response genes.
 The Journal of biological chemistry (Sakamoto S, Potla R, Larner AC) 279/39, 40362-40367 200409 () 10.1074/jbc.M406400200

Identification of a novel conserved motif in the STAT family that is required for tyrosine phosphorylation.
 The Journal of biological chemistry (Gamero AM, Sakamoto S, Montenegro J, Larner AC) 279/13, 12379-12385 200403 () 10.1074/jbc.M310787200

Cells previously desensitized to type 1 interferons display different mechanisms of activation of stat-dependent gene expression from na?ve cells.
 The Journal of biological chemistry (Sakamoto S, Qin J, Navarro A, Gamero A, Potla R, Yi T, Zhu W, Baker DP, Feldman G, Larner AC) 279/5, 3245-3253 200401 () 10.1074/jbc.M309631200

Pretreatment of Na?ve Cells with IFNg Leads to a Significant Transcrptional Activation of the Lipopolysaccharide (LPS) Inducible Interferon Stimulated Gene-54K
 Proceeding of the 12th International Conference on Second Messengers and Phosphoproteins (Sakamoto S, Larner AC) /, 2004 ()

Inhibition of poly(ADP-ribose) polymerase activity by Bcl-2 in association with the ribosomal protein S3a.
 Biochemistry (Song D, Sakamoto S, Taniguchi T) 41/3, 929-934 200201 () 10.1021/bi015669c

Genetic background determines sensitivity to the inhibitory function of NO on T cell proliferation and the amounts of NO production mediated through IFN-gamma.
 Microbiology and immunology (Ozaki A, Fukushima A, Fukata K, Sakamoto S, Taniguchi T, Hashiguch M, Ueno H) 46/8, 555-563 2002 ()

Cloning, sequencing, and expression of the meso-diaminopimelate dehydrogenase gene from Bacillus sphaericus.
 Journal of Molecular Catalysis: B:Enzymatic (Sakamoto S, Seki M, Nagata S, Misono H) 12/, 85-92 2001 () 10.1016/S1381-1177(00)00207-1

Mechanism for maintenance of dominant T helper 1 immune responses in Lewis rats.
 Microbiology and Immunology (Sakamoto S, Fukushima A, Ozaki A, Ueno H, Kamakura M, Taniguchi T) 45/5, 373-381 2001 ()

Identification of a phorbol ester-responsive element in the IFNg receptor 1 chain gene
 The Journal of Biological Chemistry 高知医科大 (現 高知大学 医学部) (Sakamoto S, Taniguchi T) 276/40, 37237-37241 2001 () 10.1074/jbc.M105543200

Molecular Characterization and Nuclear Localization of Rat timeless-like Gene Product
 Biochemical and Biophysical Research Communications (Sakamoto S, Miyazaki K, Fukui H, Oishi K, Hayasaka N, Okada M, Kamakura M, Taniguchi T, Nagai K, Ishida N) 279/, 131-138 2000 () 10.1006/bbrc.2000.3927

A Binding Protein to the DNase I Hypersensitive Site II in HLA-DR a Gene Was Identified as NF90.
 Biochemistry (Sakamoto S, Morisawa K, Ota K, Nie J, Taniguchi T) 38/11, 3355-3361 1999 () 10.1021/bi982099g

Phorbol Ester Induction of IFNg Receptors Leads to Enhanced DR alpha Gene Expression.
 The Journal of Immunology (Sakamoto S, Nie J, Taniguchi T) 162/, 4381-4384 1999 ()

Identification of Oct-1 and automodification domain of poly (ADP-ribose) synthetase
 FEBS Letters (Nie J, Sakamoto S, Song D, Qu Z, Ota K, Taniguchi T) 424/, 27-32 1998 () 10.1016/S0014-5793(98)00131-8

Identification of Carbonic Anhydrase IV and VI mRNA Expression in Human Pancreas and Salivary Glands.
 Pancreas (Fujikawa-Adachi K, Nishimori I, Sakamoto S, Morita M, Onishi S, Yonezawa S, Hollingsworth.A M) 18/4, 329-335 1999 ()

microRNA生合成変動が引き起こす病態生理現象
 比較生理生化学 (戸高 寛、樋口 琢磨、坂本 修士) 33/4, 183-190 2016 () 10.3330/hikakuseiriseika.33.183

マルチPK抗体とPhos-tagを利用した細胞内リン酸化動態の新規解析法
 生物物理化学 (杉山 康憲、亀下 勇、坂本 修士) 59/2, 82-84 2015 () 10.2198/electroph.59.82

microRNA生合成における負の制御
 THE LUNG perspectives (坂本 修士、樋口 琢磨、戸高 寛) 19/3, 96-103 2011 ()

■ 研究業績(著書)
PCR実験 なるほどQ and A(2011)
 出版社:羊土社

改訂PCR実験ノート(2005)
 出版社:羊土社

遺伝子工学がわかる(2001)
 出版社:羊土社

■ 研究業績(口頭・ポスター)

■ 研究業績(特許)

■ 研究業績(その他の活動)


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