■ 氏名
杉山 成 (スギヤマ シゲル)
SUGIYAMA Shigeru

■ 所属部局/職名
Research and Education Faculty, Natural Sciences Cluster, Science and Technology Unit/Professor
教育研究部 自然科学系 理工学部門/教授

■ 学部・学科・コース等
Faculty of Science and Technology
理工学部 化学生命理工学科

■ 連絡先
住 所高知県高知市曙町2-5-1
電 話088-844-8286
F A X
E-Mailssugiyama●kochi-u.ac.jp    (送信の際は“●”を半角の“@”に変更して送信してください。)
U R L

■ 出身大学院・研究科等
姫路工業大学 修士(工学研究科 応用化学専攻) 1992 (修了)

■ 出身学校・専攻等
姫路工業大学 (工学部 応用化学科) 1990 (卒業)

■ 取得学位
博士(工学) (論文) 大阪大学 構造生物化学

■ 研究職歴
1992年 4月 協和発酵工業株式会社 医薬研究所 研究員
1992年 5月 株式会社蛋白工学研究所へ出向 第一研究部 研究員
1995年 8月 協和発酵工業株式会社 医薬研究所 研究員
1997年 8月 株式会社丸和栄養食品 取締役研究開発部長
2005年10月 有限会社モルロジックス 取締役
2007年 4月 大阪大学大学院工学研究科 特任研究員(客員准教授)
2011年 4月 大阪大学大学院理学研究科 特任准教授
2017年 4月 高知大学教育研究部自然科学系理学部門 教授
       大阪大学大学院 招聘教授

■ 委員歴・役員歴

■ 受賞学術賞
応用物理学会 講演奨励賞 (2015)
レーザー学会 論文発表奨励賞 (2014)
大阪大学総長表彰 (2013)
日本結晶成長学会 第29回論文賞 (2012)
日本農芸化学会 トピックス賞 (2011)
13th International Conference on the Crystallization of Biological Macromolecules (ICCBM13), Poster Prize Winner (2010) 国外

■ 所属学会
日本蛋白質科学会
 ( 国内)
日本結晶学会
 (年会プログラム委員 H29 国内)
日本結晶成長学会
 ( 国内)
日本生物物理学会
 (分野別専門委員 H26, H28, H30 国内)
高分子学会
 ( 国内)
日本ポリアミン学会
 ( 国内)

■ 専門分野
タンパク質結晶学、構造生物化学

■ 主な研究テーマ・活動

■ 研究についてのPR

■ 社会人・生徒を対象として可能な講演・授業(例えば講演題目)
バイオベンチャーと宇宙ビジネス

■ 研究業績(論文・解説)
Characterization of an aptamer against Runt domain of AML1 (RUNX1) by NMR and mutational analyses
 FEBS Open Bio (Kenta Takada, Ryo Amano, Yusuke Nomura, Yoichiro Tanaka, Shigeru Sugiyama, Takashi Nagata, Masato Katahira, Yoshikazu Nakamura, Gota Kawai, Tomoko Kozu, and Taiichi Sakamoto) 8/2, 264-270 2018 (学術雑誌) 10.1002/2211-5463.12368

脂質結合タンパク質の分子認識機構−水と油の相性は?
 月刊「化学」 (杉山成、原利明、松岳大輔、松岡茂、村田道雄) 73/2, 23-27 2018 (学術雑誌)

Improving the solubility of artificial ligands of streptavidin to enable more practical reversible switching of protein localization in cells
 ChemBioChem (Ryo Tachibana, Takuya Terai, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Franck Perez, Yasuteru Urano) 18/4, 358-362 2017 (学術雑誌) 10.1002/cbic.201600640

Crystallization of acetaminophen form II by plastic-ball-assisted ultrasonic irradiation
 Appl. Phys. Express (Yoichiro Mori, Mihoko Maruyama, Yoshinori Takahashi, Hiroshi Y. Yoshikawa, Shino Okada, Hiroaki Adachi, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, Masashi Yoshimura, Yusuke Mori) 10/2, 025501 (4 pages) 2017 (学術雑誌) 10.7567/APEX.10.025501

Metastable crystal growth of acetaminophen using solution-mediated phase transformation
 Appl. Phys. Express (Yoichiro Mori, Mihoko Maruyama, Yoshinori Takahashi, Hiroshi Y. Yoshikawa, Shino Okada, Hiroaki Adachi, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, Masashi Yoshimura, and Yusuke Mori) 10/1, 015501 (3 pages) 2017 (学術雑誌) 10.7567/APEX.10.015501

Genetically engineered monomeric streptavidin for designing a new detection systems based on monovalent biotin interaction
 The ShujitusUniversity Jounal of Pharmaceutical Sciences (Osamu Hiraoka, Takuya Terai, Moe Kohno, Shigeru Sugiyama) 3/1, 16-26 2016 (大学・研究所等紀要)

Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent
 Proc Natl Acad Sci USA (Takanori Nakane, Shinya Hanashima, Mamoru Suzuki, Haruka Saiki, Taichi Hayashi, Keisuke Kakinouchi, Shigeru Sugiyama, Satoshi Kawatake, Shigeru Matsuoka, Nobuaki Matsumori, Eriko Nango, Jun Kobayashi, Tatsuro Shimamura, Kanako Kimura, Chihiro Mori, Naoki Kunishima, Michihiro Sugahara, Yoko Takakyu, Shigeyuki Inoue, Tetsuya Masuda, Toshiaki Hosaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Tsuyoshi Inoue, Osamu Nureki, So Iwata, Michio Murata, and Eiichi Mizohata) 113/46, 13039-13044 2016 (学術雑誌) 10.1073/pnas.1602531113

Promotion of protein crystal growth by actively switching crystal growth mode via femtosecond laser ablation
 Nat. Photonics (Yusuke Tominaga, Mihoko Maruyama, Masashi Yoshimura, Haruhiko Koizumi, Masaru Tachibana, Shigeru Sugiyama, Hiroaki Adachi, Katsuo Tsukamoto, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Hiroshi Y. Yoshikawa, and Yusuke Mori) 10/11, 723-726 2016 (学術雑誌) 10.1038/NPHOTON.2016.202

A crystallization technique for obtaining large protein crystals with increased mechanical stability using agarose gel combined with a stirring technique
 J. Cryst. Growth (Mihoko Maruyama, Yuki Hayashi, Hiroshi Y. Yoshikawa, Shino Okada, Haruhiko Koizumi, Masaru Tachibana, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Satoshi Murakami, Masashi Yoshimura, and Yusuke Mori) 452/, 172-178 2016 (学術雑誌) 10.1016/j.jcrysgro.2015.11.008

Molecular mechanism underlying promiscuous polyamine recognition by spermidine acetyltransferase
 Int. J. Biochem. Cell Biol. (Shigeru Sugiyama*, Sae Ishikawa, Hideyuki Tomitori, Mayumi Niiyama, Mika Hirose, Yuma Miyazaki, Kyohei Higashi, Michio Murata, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Keiko Kashiwagi, Kazuei Igarashi, and Hiroyoshi Matsumura) 76/6, 87-97 2016 (学術雑誌) 10.1016/j.biocel.2016.05.003

Protein crystallization with paper
 Jpn. J. Appl. Phys. (Miki Matsuoka, Keisuke Kakinouchi, Hiroaki Adachi, Mihoko Maruyama, Shigeru Sugiyama, Satoshi Sano, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Masashi Yoshimura, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano) 55/5, 050302 (3 pages) 2016 (学術雑誌) 10.7567/JJAP.55.050302

Growth of high-strength protein crystals with nanofibers
 Appl. Phys. Express (Miki Matsuoka, Keisuke Kakinouchi, Hiroaki Adachi, Mihoko Maruyama, Shigeru Sugiyama, Iori Nakabayashi, Hiroshi Tsuchikura, Atsushi Kuwahara, Satoshi Sano, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Masashi Yoshimura, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano) 9/3, 035503 (3 pages) 2016 (学術雑誌) 10.7567/APEX.9.035503

Development of protein seed crystals reinforced with high-strength hydrogels
 CrystEngComm (Shigeru Sugiyama*, Noriko Shimizu, Keisuke Kakinouchi, Osamu Hiraoka, Hiroyoshi Matsumura, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Masashi Yoshimura, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Michio Murata, and Yusuke Mori) 17/42, 8064-8071 2015 (学術雑誌) 10.1039/c5ce00844a

Artificial ligands of streptavidin (ALiS): Discovery, characterization, and application for reversible control of intracellular protein transport
 J. Am. Chem. Soc. (Takuya Terai, Moe Kohno, Gaelle Boncompain, Shigeru Sugiyama, Nae Saito, Ryo Fujikake, Tasuku Ueno, Toru Komatsu, Kenjiro Hanaoka, Takayoshi Okabe, Yasuteru Urano, Franck Perez, and Tetsuo Nagano) 137/33, 10464-10467 2015 (学術雑誌) 10.1021/jacs.5b05672

Bioactive structure of membrane lipids and natural products elucidated by a chemistry-based approach
 Chemical Record (Michio Murata, Shigeru Sugiyama, Shigeru Matsuoka, and Nobuaki Matsumori) 15/4, 675-690 2015 (学術雑誌) 10.1002/tcr.201402097

Selective crystallization of metastable phase of acetaminophen by ultrasonic irradiation
 Appl. Phys. Express (Yoichiro Mori, Mihoko Maruyama, Yoshinori Takahashi, Kenji Ikeda, Suguru Fukukita, Hiroshi Y. Yoshikawa, Shino Okada, Hiroaki Adachi, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, Masashi Yoshimura, and Yusuke Mori) 8/6, 065501 (4 pages) 2015 (学術雑誌) 10.7567/APEX.8.065501

Selective crystallization of the metastable phase of indomethacin at the interface of liquid/air bubble induced by femtosecond laser irradiation
 Appl. Phys. Express (Kenji Ikeda, Mihoko Maruyama, Yoshinori Takahashi, Yoichiro Mori, Hiroshi Y. Yoshikawa, Shino Okada, Hiroaki Adachi, Shigeru Sugiyama, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, Masashi Yoshimura, and Yusuke Mori) 8/4, 045501 (4 pages) 2015 (学術雑誌)

Spiral growth can enhance the both of normal growth rate and crystal quality in tetragonal lysozyme growth under a forced solution flow
 Cryst. Growth Des. (Yuki Hayashi, Mihoko Maruyama*, Masashi Yoshimura, Shino Okada, Hiroshi Yoshikawa, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Satoshi Murakami, and Yusuke Mori) 15/5, 2137-2143 2015 (学術雑誌) 10.1021/cg500484e

A comparative analysis of microgravity and earth grown thermostable T1 lipase crystals using HDPCG apparatus
 Protein Pept. Lett. (Raja Noor Zaliha Raja Abd Rahman, Mohd. Shukuri Mohamad Ali, Shigeru Sugiyama, Adam Thean Chor Leow, Tsuyoshi Inoue, Mahiran Basri, Abu Bakar Salleh and Hiroyoshi Matsumura) 22/2, 173-179 2015 (学術雑誌) 10.2174/0929866521666141019193604

Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of seed storage protein
 Acta Crystallogr. F (Nobuyuki Maruyama, Tomoriho Goshi, Shigeru Sugiyama, Mayumi Niiyama, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Hiroyoshi Matsumura and Bunzo Mikami) 71/2, 132-135 2015 (学術雑誌) 10.1107/S2053230X14027484

Molecular dynamics simulations of heart-type fatty acid binding protein in apo and holo forms, and hydration structure analyses in the binding cavity
 J. Phys. Chem. B (Daisuke Matsuoka, Shigeru Sugiyama, Michio Murata and Shigeru Matsuoka) 119/1, 114-127 2015 (学術雑誌) 10.1021/jp510384f

Water-mediated recognition of simple alkyl chains by heart-type fatty acid binding protein
 Angew. Chem. Int. Ed. (Shigeru Matsuoka†, Shigeru Sugiyama†, Daisuke Matsuoka, Mika Hirose, Lethu Sebastien, Hikaru Ano, Toshiaki Hara, Osamu Ichihara, Roy Kimura, Satoshi Murakami, Hanako Ishida, Eiichi Mizohata, Tsuyoshi Inoue, and Michio Murata (†These two authors contributed equally to this work.)) 54/5, 1508-1511 2015 (学術雑誌) 10.1002/anie.201409830

Grease matrix as a versatile carrier of proteins for serial crystallography
 Nat. Method (Michihiro Sugahara, Eiichi Mizohata, Eriko Nango, Mamoru Suzuki, Tomoyuki Tanaka, Tetsuya Masuda, Rie Tanaka, Tatsuro Shimamura, Yoshiki Tanaka, Chiyo Suno, Kentaro Ihara, Dongqing Pan, Keisuke Kakinouchi, Shigeru Sugiyama, Michio Murata, Tsuyoshi Inoue, Kensuke Tono, Changyong Song, Jaehyun Park, Takashi Kameshima, Takaki Hatsui, Yasumasa Joti, Makina Yabashi, So Iwata) 12/1, 61-63 2015 (学術雑誌) 10.1038/nmeth.3172

Functional expression and characterization of EF-hand endoplasmic reticulum-resident calcium-binding protein, PfERC from Plasmodium in Escherichia coli for crystallization
 The ShujitusUniversity Jounal of Pharmaceutical Sciences (Osamu Hiraoka, Yasuhide Takashima, Sayuri Sakamoto, Shigeru Sugiyama, Masayuki Morita, Yusuke Wataya, and Hye-Sook Kim) 1/1, 42-50 2014 (大学・研究所等紀要)

Crystallization and preliminary crystallographic studies of PotA, a membrane-associated ATPase of the spermidine-preferential uptake system in Thermotoga maritima
 Acta Crystallogr. F (Shigeru Sugiyama*, Keiko Kashiwagi, Keisuke Kakinouchi, Hideyuki Tomitori, Ken Kanai, Michio Murata, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori and Kazuei Igarashi) 70/6, 738-741 2014 (学術雑誌) 10.1107/S2053230X14008607

A new practical technique for high quality protein crystallization with the solution stirring technique at the interface between high-concentrated hydrogel and solution
 Jpn. J. Appl. Phys (Yusuke Aoki, Mihoko Maruyama, Yoshinori Takahashi, Masashi Yoshimura, Hiroshi Y. Yoshikawa, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Hiroyoshi Matsumura, Tsuyoshi Inoue, and Yusuke Mori) 53/6, 065502 (6 pages) 2014 (学術雑誌)

Interaction kinetics of liposome-incorporated unsaturated fatty acids with fatty acid-binding protein 3 by surface plasmon resonance
 Bioorgan. Med. Chem. (Maria Carmen Tan, Shigeru Matsuoka, Hikaru Ano, Hanako Ishida, Mika Hirose, Fuminori Satou, Shigeru Sugiyama, and Michio Murata) 22/6, 1804-1808 2014 (学術雑誌) 10.1016/j.bmc.2014.02.001

Laser ablation for protein crystal nucleation and seeding
 Chemical Society Reviews (Hiroshi Y. Yoshikawa, Ryota Murai, Hiroaki Adachi, Shigeru Sugiyama, Mihoko Maruyama, Yoshinori Takahashi, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Hiroshi Masuhara, and Yusuke Mori) 43/7, 2147-2158 2014 (学術雑誌) 10.1039/C3CS60226E

凝固したハイドロゲル中でタンパク質結晶を育成させる新規結晶化技術を開発
 高分子 (杉山成、清水典子、佐崎元、廣瀬未果、高橋義典、丸山美帆子、松村浩由、安達宏昭、高野和文、村上聡、井上豪、森勇介) 62/743, 759 2013 (学術雑誌)

溶液流れ下におけるタンパク質の結晶成長
 日本結晶成長学会 (丸山美帆子、佐崎元、高橋義典、吉村政志、吉川洋史、杉山成、安達宏昭、松村浩由、井上豪、高野和文、村上聡、森勇介) 40/2, 72-82 2013 (学術雑誌)

Structure of the human-heart fatty-acid-binding protein 3 in complex with the fluorescent probe 1-anilinonaphtalene-8-sulphonic acid
 J. Synchrotron Radiat. (Mika Hirose, Shigeru Sugiyama*, Hanako Ishida, Mayumi Niiyama, Daisuke Matsuoka, Toshiaki Hara, Eiichi Mizohata, Satoshi Murakami, Tsuyoshi Inoue, Shigeru Matsuoka, and Michio Murata) 20/6, 923-928 2013 (学術雑誌) 10.1107/S0909049513021298

Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli
 Acta Crystallogr. F (Mayumi Niiyama, Shigeru Sugiyama*, Mika Hirose, Sae Ishikawa, Hideyuki Tomitori, Kyohei Higashi, Tomoko Yamashita, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Michio Murata, Tsuyoshi Inoue, Yusuke Mori, Keiko Kashiwagi, Hiroyoshi Matsumura, and Kazuei Igarashi) 69/8, 884-887 2013 (学術雑誌)

A novel approach for protein crystallization by a synthetic hydrogel with thermoreversible gelation polymer
 Cryst. Growth Des. (Shigeru Sugiyama*, Noriko Shimizu, Gen Sazaki, Mika Hirose, Yoshinori Takahashi, Mihoko Maruyama, Hiroyoshi Matsumura, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori) 13/5, 1899-1904 2013 (学術雑誌) 10.1021/cg301588b

Effect of gel-solution interface on femtosecond laser-induced nucleation of protein
 Cryst. Growth Des. (Satoshi Nakayama, Hiroshi Yoshikawa, Ryota Murai, Masateru Kurata, Mihoko Maruyama, Shigeru Sugiyama, Yusuke Aoki, Yoshinori Takahashi, Masashi Yoshimura, Seiichiro Nakabayashi, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Satoshi Murakami, Yusuke Mori) 13/4, 1491-1496 2013 (学術雑誌)

Desigh and Practice of New Style Education Class - Internship at World-Leading Science Laboratory
 OSAKA UNIVERSITY HIGHER EDUCATION STUDIES (Shigeru Sugiyama, Yshiaki Hara, Satoshi Kawatake, Masanao Kinoshita, Kazuya Yamaguchi, and Michio Murata) 1/, 59-65 2012 (その他)

固相ハイドロゲル中でのタンパク質結晶化
 日本結晶学会 (杉山成、松村浩由、丸山美帆子、佐崎元、廣瀬未果、安達宏昭、高野和文、村上聡、井上豪、森勇介) 54/5, 300-303 2012 (学術雑誌)

固相ゲル中にタンパク質結晶を育成
 化学 (杉山成) 67/9, 74-75 2012 (その他)

超高分解能光断層計測を活用し,タンパク質結晶の3次元・非破壊断層イメージングに成功
 O plus E (西澤典彦、石田周太郎、松村浩由、廣瀬未果、杉山成、森勇介、井上豪、伊東一良) 34/7, 575-576 2012 (その他)

Crystal structure of the superfamily 1 helicase from Tomato mosaic virus
 Journal of Virology (Masaki Nishikiori†, Shigeru Sugiyama†, Hongyu Xiang, Mayumi Niiyama, Kazuhiro Ishibashi, Tsuyoshi Inoue, Masayuki Ishikawa, Hiroyoshi Matsumura, and Etsuko Katoh (†These two authors contributed equally to this work.)) 86/14, 7565-7576 2012 (学術雑誌)

Effects of a forced solution flow on the step advancement on {110} faces of tetragonal lysozyme crystals: direct visualization of individual steps under a forced solution flow
 Cryst. Growth Des. (Mihoko Maruyama, Hisato Kawahara, Gen Sazaki, Shou Maki, Yoshinori Takahashi, Hiroshi Yoshikawa, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, and Yusuke Mori) 12/6, 2856-2863 2012 (学術雑誌)

Growth of protein crystals in hydrogels prevents osmotic shock
 J. Am. Chem. Soc. (Shigeru Sugiyama*, Mihoko Maruyama, Gen Sazaki, Mika Hirose, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, and Hiroyoshi Matsumura) 134/13, 5786-5789 2012 (学術雑誌)

Generation of gaseous sulfur-containing compounds in tumor tissue and suppression of gas diffusion as an antitumor treatment
 Gut (Kazue Yamagishi, Kazuo Onuma, Yota Chiba, Shinya Yagi, Shigenobu Aoki, Tomoyuki Sato, Yasushi Sugawara, Noriyasu Hosoya, Yasutake Saeki, Minoru Takahashi, Masayoshi Fuji, Takeo Ohsaka, Takeyoshi Okajima, Kenji Akita, Takashi Suzuki, Pisol Senawongse, Akio Urushiyama, Kiyoshi Kawai, Hirofumi Shoun, Yoshimasa Ishii, Hiroya Ishikawa, Shigeru Sugiyama, Madoka Nakajima, Masaru Tsuboi, and Tateo Yamanaka) 61/4, 554-561 2012 (学術雑誌) 10.1136/gutjnl-2011-300721

Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography
 Biomedical Optics Express (Norihiko Nishizawa, Shutaro Ishida, Mika Hirose, Shigeru Sugiyama, Tsuyoshi Inoue, Yusuke Mori, Kazuyoshi Itoh, and Hiroyoshi Matsumura) 3/4, 735-740 2012 (学術雑誌)

フェムト秒レーザー誘起キャビテーションバブルによるタンパク質の結晶核発生
 日本結晶成長学会 (吉川洋史、村井良多、杉山成、安達宏昭、松村浩由、井上豪、村上聡、高野和文、森勇介) 38/3, 161-168 2011 (学術雑誌)

結晶成長の新技術
 バイオインダストリー (松村浩由、杉山成、安達宏昭、高野和文、村上聡、井上豪、森勇介) 28/3, 30-36 2011 (学術雑誌)

Crystallization and preliminary X-ray crystallographic analysis of a helicase-like domain from a tomato mosaic virus replication protein
 Acta Crystallogr. F (Hongyu Xiang, Mayumi Niiyama, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Masayuki Ishikawa, Hiroyoshi Matsumura, and Etsuko Katoh) 67/12, 1649-1652 2011 (学術雑誌)

Structural insight into the stereoselective production of PGF2α by Old Yellow Enzyme from Trypanosoma cruzi
 J. Biochem. (Naoki Okamoto, Keishi Yamaguchi, Eiichi Mizohata, Keiji Tokuoka, Nahoko Uchiyama, Shigeru Sugiyama, Hiroyoshi Matsumura, Koji Inaka, Yoshihiro Urade and Tsuyoshi Inoue) 150/5, 563-568 2011 (学術雑誌)

Rational development of caged-biotin protein-labeling agents and some applications in live cells
 Chem. Biol. (Takuya Terai, Eri Maki, Shigeru Sugiyama, Yoshinori Takahashi, Hiroyoshi Matsumura, Yusuke Mori, and Tetsuo Nagano) 18/10, 1261-1272 2011 (学術雑誌)

Influence of energy and wavelength on femtosecond laser-induced nucleation of protein
 Chemical Physics Letters (Ryota Murai, Hiroshi Y. Yoshikawa, Hitoshi Hasenaka, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori) 510/1-3, 139-142 2011 (学術雑誌)

Growth of protein crystals by syringe-type top-seeded solution growth
 Cryst. Growth Des. (Hiroyoshi Matsumura, Keisuke Kakinouchi, Tsutomu Nakamura, Shigeru Sugiyama, Mihoko Maruyama, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami Tsuyoshi Inoue and Yusuke Mori) 11/5, 1486-1492 2011 (学術雑誌)

Laser-induced nucleation in protein crystallization: Local increase in protein concentration induced by femtosecond laser irradiation
 J. Cryst. Growth (Natsuko Iefuji, Ryota Murai, Mihoko Maruyama, Yoshinori Takahashi, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yuichi Koga, Kazufumi Takano and Shigenori Kanaya) 318/1, 741-744 2011 (学術雑誌)

Effect of evaporation on protein crystals grown in semi-solid agarose hydrogel
 Jpn. J. Appl. Phys. (Shigeru Sugiyama, Mika Hirose, Noriko Shimizu, Mayumi Niiyama, Mihoko Maruyama, Gen Sazaki, Ryota Murai, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori and Hiroyoshi Matsumura) 50/2, 025502 (4 pages) 2011 (学術雑誌)

Approach for growth of high-quality and large protein crystals
 J. Synchrotron Radiat. (Hiroyoshi Matsumura, Shigeru Sugiyama, Mika Hirose, Keisuke Kakinouchi, Mihoko Maruyama, Ryota Murai, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori and Tsuyoshi Inoue) 18/1, 16-19 2011 (学術雑誌)

Structure of the inhibitor complex of old yellow enzyme from Trypanosoma cruzi
 J. Synchrotron Radiat. (Keishi Yamaguchi, Naoki Okamoto, Keiji Tokuoka, Shigeru Sugiyama, Nahoko Uchiyama, Hiroyoshi Matsumura, Koji Inaka, Yoshihiro Urade and Tsuyoshi Inoue) 18/1, 66-69 2011 (学術雑誌)

Conformational plasticity of RNA for target recognition as revealed by the 2.15 A crystal structure of a human IgG-aptamer complex
 Nucleic Acids Res. (Yusuke Nomura†, Shigeru Sugiyama†, Taiichi Sakamoto, Shin Miyakawa, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yoshikazu Nakamura and Hiroyoshi Matsumura (†These two authors contributed equally to this work.)) 38/21, 7822-7829 2010 (学術雑誌)

Estimated effects of silicone glue on protein crystal growth
 J. Cryst. Growth (Mihoko Maruyama, Noriko Shimizu, Shigeru Sugiyama, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Hiroyoshi Matsumura and Yusuke Mori) 312/19, 2771-2774 2010 (学術雑誌)

Growth of large protein crystals by a large-scale hanging drop method
 J. Appl. Cryst. (Keisuke Kakinouchi, Tsutomu Nakamura, Taro Tamada, Hiroaki Adachi, Shigeru Sugiyama, Mihoko Maruyama, Yoshinori Takahashi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki, Yusuke Mori, and Hiroyoshi Matsumura) 43/4, 937-939 2010 (学術雑誌)

Crystal growth procedure of HIV-1 protease-inhibitor KNI-272 complex for neutron structural analysis at 1.9 A resolution
 Cryst. Growth Des. (Noriko Shimizu, Shigeru Sugiyama, Mihoko Maruyama, Yoshinori Takahashi, Motoyasu Adachi, Taro Tamada, Koushi Hidaka, Yoshio Hayashi, Tooru Kimura, Yoshiaki Kiso, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki, Yusuke Mori and Hiroyoshi Matsumura) 10/7, 2990-2994 2010 (学術雑誌)

Enhancement of femtosecond laser-induced nucleation of protein in a gel solution
 Appl. Phys. Lett. (Ryota Murai, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, and Yusuke Mori) 96/4, 043702 (3 pages) 2010 (学術雑誌)

Femtosecond laser processing of protein crystals grown in agarose gel
 J. Cryst. Growth (Hitoshi Hasenaka†, Shigeru Sugiyama†, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, and Hiroyoshi Matsumura (†These two authors contributed equally to this work.)) 312/1, 73-78 2009 (学術雑誌)

Growth of large protein crystals by top-seeded solution growth together with the floating and solution-stirring technique
 Cryst. Growth Des. (Noriko Shimizu, Shigeru Sugiyama, Mihoko Maruyama, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Hiroyoshi Matsumura, and Yusuke Mori) 9 /12, 5227-5232 2009 (学術雑誌)

Promotion of crystal nucleation of protein by semi-solid agarose gel
 Appl. Phys. Express (Kana Tanabe, Mika Hirose, Ryota Murai, Shigeru Sugiyama, Noriko Shimizu, Mihoko Maruyama, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Eiichi Mizohata, Tsuyoshi Inoue, and Hiroyoshi Matsumura) 2/12, 125501 (3 pages) 2009 (学術雑誌) 10.1143/APEX.2.125501

A manipulating tool for protein microcrystals in solution using adhesive materials
 Jpn. J. Appl. Phys. (Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, and Kazufumi Takano) 48/12, 118011 (2 pages) 2009 (学術雑誌) 10.1143/JJAP.48.118001

Femtosecond laser processing of agarose gel surrounding protein crystals for development of an automated crystal capturing system
 Jpn. J. Appl. Phys. (Shigeru Sugiyama, Hitoshi Hasenaka, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, and Hiroyoshi Matsumura) 48/10, 105502 (5 pages) 2008 (学術雑誌)

Protein crystallization in agarose gel with high strength: Developing an automated system for protein crystallographic processes
 Jpn. J. Appl. Phys. (Shigeru Sugiyama, Kana Tanabe, Mika Hirose, Tomoya Kitatani, Hitoshi Hasenaka, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Tsuyoshi Inoue, and Hiroyoshi Matsumura) 48/7, 075502 (5 pages) 2009 (学術雑誌) 10.1143/JJAP.48.075502

Structure of HIV-1 protease in complex with potent inhibitor KNI-272 dDetermined by high-resolution X-ray and neutron crystallography
 Proc Natl Acad Sci USA (Motoyasu Adachi, Takashi Ohhara, Kazuo Kurihara, Taro Tamada, Eijiro Honjo, Nobuo Okazaki, Shigeki Arai, Yoshinari Shoyama, Kaname Kimura, Hiroyoshi Matsumura, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano, Yusuke Mori, Koushi Hidaka, Tooru Kimura, Yoshio Hayashi, Yoshiaki Kiso, and Ryota Kuroki) 106/12, 4641-4646 2009 (学術雑誌)

Femtosecond laser-induced nucleation of protein in agarose gel
 J. Cryst. Growth (Hiroshi Y. Yoshikawa, Ryota Murai, Shigeru Sugiyama, Gen Sazaki, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Kazufumi Takano, and Yusuke Mori) 311/3, 956-959 2009 (学術雑誌)

Evaluation and improvement of a technique to manipulate protein crystals in solution
 Jpn. J. Appl. Phys. (Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, and Kazufumi Takano) 47/12, 8995-8997 2008 (学術雑誌) 10.1143/JJAP.47.8995

Crystallization and preliminary X-ray crystallographic analysis of Ca2+-free primary Ca2+-sensor of Na+/Ca2+ exchanger
 Acta Crystallogr. F (Masashi Mima, Chika Kawai, Kumsun Paku, Koji Tomoo, Toshimasa Ishida, Shigeru Sugiyama, Hiroyoshi Matsumura, Tomoya Kitatani, Hiroshi Y. Yoshikawa, Syou Maki, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Satomi Kita, and Takahiro Iwamoto) 64/12, 1125-1127 2008 (学術雑誌)

Laser energy dependence on femtosecond laser-induced nucleation of protein
 Applied Physics A (Hiroshi Y. Yoshikawa, Ryota Murai, Syou Maki, Tomoya Kitatani, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Tsuyoshi Inoue, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Takatomo Sasaki and Yusuke Mori) 93/4, 911-915 2008 (学術雑誌)

Crystallization and preliminary neutron diffraction studies of HIV-1 protease cocrystalized with inhibitor KNI-272
 Acta Crystallogr. F (Hiroyoshi Matsumura, Motoyasu Adachi, Shigeru Sugiyama, Shino Okada, Megumi Yamakami, Taro Tamada, K. Hidaka, Y. Hayashi, Y. Kiso, Syou Maki, Tomoya Kitatani, Hiroshi Y. Yoshikawa, Asako Kobayashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki and Yusuke Mori) 64/11, 1003-1006 2008 (学術雑誌)

Crystallization and preliminary X-ray diffraction studies of an RNA aptamer in complex with the human IgG Fc fragment
 Acta Crystallogr. F (Shigeru Sugiyama, Yusuke Nomura, Taiichi Sakamoto, Tomoya Kitatani, Asako Kobayashi, Shin Miyakawa, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yoshikazu Nakamura, and Hiroyoshi Matsumura) 64/10, 942-944 2008 (学術雑誌)

Protein crystallization in 100 nl solution with new stirring equipment
 J. Synchrotron Radiat. (Syou Maki, Ryota Murai, Hiroshi Y. Yoshikawa, Tomoya Kitatani, Shinya Nakata, Hisato Kawahara, Hitoshi Hasenaka, Asako Kobayashi, Shino Okada, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Takatomo Sasaki and Yusuke Mori) 15/3, 269-272 2008 (学術雑誌)

Effect of solution flow produced by rotary shaker on protein crystallization
 J. Cryst. Growth (Ryota Murai, Hiroshi Y. Yoshikawa, Hisato Kawahara, Syou Maki, Shigeru Sugiyama, Tomoya Kitatani, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Takatomo Sasaki and Yusuke Mori) 310/7-9, 2168-2172 2008 (学術雑誌)

New technique of manipulating a protein crystal using adhesive material
 Appl. Phys. Express (Tomoya Kitatani, Shigeru Sugiyama, Hiroyoshi Matsumura, Hiroaki Adachi, Hiroshi Y. Yoshikawa, Syou Maki, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori and Kazufumi Takano) 1/7, 037002 (3 pages) 2008 (学術雑誌) 10.1143/APEX.1.037002

フェムト秒レーザーによるタンパク質結晶化の制御
 レーザー研究 (吉川洋史、村井良多、牧祥、北谷友也、杉山成、安達宏昭、高野和文、松村浩由、村上聡、井上豪、佐々木孝友、森勇介) /, 7-11 2007 (その他)

Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi.
 Acta Crystallogr. F (Shigeru Sugiyama, Keiji Tokuoka, NahokoUchiyama, Naoki Okamoto, Yousuke Okano, Hiroyoshi Matsumura, Koji Inaka, Yoshihiro Urade and Tsuyoshi Inoue) 63/10, 896-898 2007 (学術雑誌)

Crystal structures of human dipeptidyl peptidase IV in its apo and diprotin B-complexed forms
 Acta Biochim Biophys Sin. (Hajime Hiramatsu, Kiyoshi Kyono, Atsushi Yamamoto, Kazuhiko Saeki, Hideaki Shima, Shigeru Sugiyama, Koji Inaka and Ryo Shimizu) 39/5, 335-343 2007 (学術雑誌)

Crystallization of the archaeal transcription termination factor NusA: a significant decrease in twinning under microgravity conditions
 Acta Crystallogr. F (Hiroaki Tanaka, Takashi Umehara, Koji Inaka, Sachiko Takahashi, Rie Shibata, Yoshitaka Bessho, Masaru Sato, Shigeru Sugiyama, Emiko Fusatomi, Takaho Terada, Mikako Shirouzu, Satoshi Sano, Moritoshi Motohara, Tomoyuki Kobayashi, Tetsuo Tanaka, Akiko Tanaka, and Shigeyuki Yokoyama) 63/2, 69-73 2007 (学術雑誌)

JAXA-GCF project - High-quality protein crystals grown under microgravity environment for better understanding of protein structure
 Microgravity Sci. Technol. (Masaru Sato, Hiroaki Tanaka, Koji Inaka, Shinichi Shinozaki, Ari Yamanaka, Sachiko Takahashi, Mari Yamanaka, Eriko Hirota, Shigeru Sugiyama, Mitsuyasu Kato, Chie Saito, Satoshi Sano, Moritoshi Motohara, Tai Nakamura, Tomoyuki Kobayashi, Susumu Yoshitomi, and Tetsuo Tanaka) 18/3-4, 184-189 2006 (学術雑誌)

Structural and mutational analysis of Trypanosoma brucei prostaglandin H2 reductase provides insight into the catalytic mechanism of aldo-ketoreductases
 J. Biol. Chem. (Kubata B. Kilunga, Tsuyoshi Inoue, Yousuke Okano, Zakayi Kabututu, Samuel K. Martin, Machael Lazarus, Michael Duzenko, Yuichi Sumii, Yukiko Kusakari, Hiroyoshi Matsumura, Yasushi Kai, Shigeru Sugiyama, Koji Inaka, Takashi Inui, and Yoshihiro Urade) 280/28, 26371-26382 2005 (学術雑誌)

Effects of different anti-tau antibodies on tau fibrillogenesis: RTA-1 and RTA-2 counteract tau aggregation
 FEBS. lett. (Taizo Taniguchi, Miho Sumida, Shuko Hiraoka, Koji Tomoo, Tomoko Kakehi, Katsuhiko Minoura, Shigeru Sugiyama, Koji Inaka, Toshimasa Ishida, Naoaki Saito and Chikako Tanaka) 579/6, 1399-1404 2005 (学術雑誌)

Numerical analysis of the depletion zone formation around a growing protein crystal
 Ann N Y Acad Sci. (Hiroaki Tanaka, Koji Inaka, Shigeru Sugiyama, Sachiko Takahashi, Satoshi Sano, Masaru Sato and Susumu Yoshitomi) 1027/1, 10-19 2004 (学術雑誌)

The crystal structure of human dipeptidyl peptidase IV (DPPIV) complex with diprotin A
 Biol. Chem. (Hajime Hiramatsu, Atsushi Yamamoto, Kiyoshi Kyono, Yutaka Higashiyama, Chiaki Fukushima, Hideaki Shima, Shigeru Sugiyama, Koji Inaka and Ryo Shimizu) 385/6, 561-564 2004 (学術雑誌)

A simplified counter diffusion method combined with a 1D simulation program for optimizing crystallization conditions
 J. Synchrotron Radiat. (Hiroaki Tanaka, Koji Inaka, Shigeru Sugiyama, Sachiko Takahashi, Satoshi Sano, Masaru Sato and Susumu Yoshitomi) 11/1, 45-48 2004 (学術雑誌)

The structure and funtion of human dipeptidyl peptidase IV, possessing a unique eight-bladed β-propeller fold
 Biochem. Biophys. Res. Comm. (Hajime Hiramatsu, Kiyoshi Kyono, Yutaka Higashiyama, Chiaki Fukushima, Hideaki Shima, Shigeru Sugiyama, Koji Inaka, Atsushi Yamamoto and Ryo Shimizu) 302/4, 849-854 2003 (学術雑誌)

微小重力環境を利用した蛋白質結晶化実験
 構造生物 (高橋幸子、田中広明、伊中浩治、杉山成、佐野智、本原守利、佐藤勝) 9/, 20-36 2003 (学術雑誌)

Crystallization and preliminary X-ray study of human dipeptidyl peptidase IV (DPPIV)
 Acta Crystallogr. D (Hajime Hiramatsu, Kiyoshi Kyono, Chiaki Fukushima, Shigeru Sugiyama, Koji Inaka, Atsushi Yamamoto and Ryo Shimizu) 59/3, 595-596 2003 (学術雑誌)

A potent aldose reductase inhibitor, (2S,4S)-6-fluoro-2’,5’-dioxospiro[chroman-4,4’-imidazolidine] -2-carboxamide(fidarestat):Its absolute configuration and interactions with the aldose reductase by X-ray crystallography
 J. Med. Chem. (Mitsuru Oka, Yukiharu Matsumoto, Shigeru Sugiyama, Nobuaki Tsuruta and Masaaki Matsushima) 43/12, 2479-2483 2000 (学術雑誌)

Preliminary crystallographic study of two crystal forms of rubredoxin from sulfate-reducing bacterium
 Protein Pept. Lett. (Yoshiki Higuchi, Shintaro Misaki, Shigeru Sugiyama, Yukio Morimoto, Mari Ogata, Tatsuhiko Yagi and Noritake Yasuoka) 5/3, 175-176 1998 (学術雑誌)

ポリアミン結合タンパク質(PotD)の構造と機能
 生化学 (杉山成、五十嵐一衛、森川耿右) 69/2, 113-119 1997 (学術雑誌)

The 1.8 Å x-ray structure of the Escherichia coli PotD protein complexed with spermidine and the mechanism of polyamine binding
 Protein Science (Shigeru Sugiyama, Yo Matsuo, Katsumi Maenaka, Dmitry G. Vassylyev, Masaaki Matsushima, Keiko Kashiwagi, Kazuei Igarashi and Kosuke Morikawa) 5/10, 1984-1990 1996 (学術雑誌)

Spermidine-preferential uptake system in Escherichia coli.: Identification of amino acids involved in polyamine binding in PotD protein
 J. Biol. Chem. (Keiko Kashiwagi, Rossella Pistocchi, Sanae Shibuya, Shigeru Sugiyama, Kosuke Morikawa and Kazuei Igarashi) 271/21, 12205-12208 1996 (学術雑誌)

Crystal Structure of PotD, the primary receptor of the polyamine transport system in Escherichia coli.
 J. Biol. Chem. (Shigeru Sugiyama, Dmitry G. Vassylyev, Masaaki Matsushima, Keiko Kashiwagi, Kazuei Igarashi and Kosuke Morikawa) 271/16, 9519-9525 1996 (学術雑誌)

Crystallization and preliminary X-ray analysis of the primary receptor (PotD) of the polyamine transport system in Escherichia coli.
 Acta Crystallogr. D (Shigeru Sugiyama, Masaaki Matsushima, Tomoko Saisho, Keiko Kashiwagi, Kazuei Igarashi and Kosuke Morikawa) 52/2, 416-418 1996 (学術雑誌)

Construction of a divalent cell adhesive lysozyme by introducing the Arg-Gly-Asp sequence at two sites
 FEBS lett. (Takao Yamada, Yoshimi Shimada, Atsuko Uyeda, Shigeru Sugiyama and Masakazu Kikuchi) 374/2, 262-264 1995 (学術雑誌)

On the active sites of Hydrogenase from Desulfovibrio vulgaris Miyazaki F
 Jpn. J. Appl. Phys. (Noritake Yasuoka, Shigeru Sugiyama, Yukio Morimoto, Yoshiki Higuchi, Shuichi Emura, Hironobu Maeda, Kichiro Koto, Izumi Nakai and Akira Yoshiasa) 32/, 553-555 1993 (学術雑誌) 10.7567/JJAPS.32S2.553

Structure of the nitrogen-centered thioaminyl radical dimer
 Chemistry Express (Yukio Morimoto, Shigeru Sugiyama, Yoshiki Higuchi, Akio Tanaka, Yozo Miura and Noritake Yasuoka) 6/6, 391-394 1991 (学術雑誌)

■ 研究業績(著書)
ゲルテクノロジーハンドブック−機能設計・評価・シミュレーションから製造プロセス・製品化まで (2014)
 出版社:株式会社エヌ・ティー・エス
 ISBN:978-4-86469-074-4

タンパク質結晶の最前線(2013)
 出版社:株式会社シーエムシー出版
 ISBN:978-4-7813-0929-3

タンパク質結晶の新展開−新しい育成技術から構造解析・応用研究へ(2008)
 出版社:株式会社シーエムシー出版
 ISBN:978-4-7813-0739-8

タンパク質の結晶化(2005)
 出版社:京都大学学術出版会
 ISBN:4-87698-657-6

■ 研究業績(口頭・ポスター)
(招待講演) フェムト秒レーザーアブレーションで制御するタンパク質結晶の成長機構 (JCCG-46結晶成長基礎・評価分科会シンポジウム 2017 口頭発表)

(招待講演) 脂肪酸結合タンパク質−水分子を介した疎水性リガンドの認識機構 (日本薬学会第137回年会シンポジウム 2017 口頭発表)

(招待講演) 溶液媒介相転移による医薬化合物アセトアミノフェンの準安定形成長(U) (第64回応用物理学会春季学術講演会 2017 口頭発表)

(Invited lecture) Growth of high-quality and large protein crystals by new seeding techniques (The 18th International Conference on Crystal Growth and Epitaxy (ICCGE18) 2016 口頭発表)

(招待講演) 脂肪酸結合タンパク質を用いたタンパク質-リガンド-水の構造機能と相互作用の解明 (iBIX 研究会 2016 口頭発表)

(招待講演) 大型タンパク質結晶育成に向けた種結晶の強化 (iBIX 研究会 2016 口頭発表)

(招待講演) 中性子用大型良質タンパク質結晶育成を目指して (平成28年度日本結晶学会年会シンポジウム 2016 口頭発表)

(招待講演) 夢の新薬をつくり出す構造生物学の世界 〜脂質-タンパク質の相互作用研究から〜 (高知大学DCセミナー・化学生命理工学セミナー・海洋生命・分子工学セミナー 2016 口頭発表)

(招待講演) 脂溶性化合物−標的蛋白質複合体の構造解析へ向けた取り組み (第16回日本蛋白質科学会ワークショップ 2016 口頭発表)

(Invited lecture) Growth of Protein Seed Crystals with High-Strength Hydrogels (16th International Conference on the Crystallization of Biological Macromolecules (ICCBM16) 2016 口頭発表)

(招待講演) 膜蛋白質−膜脂質の相互作用解析を目指した重元素化脂質の利用 (第53回日本生物物理学会年会シンポジウム 2015 口頭発表)

(招待講演) 難溶性化合物とタンパク質との複合体結晶解析へ向けた新しい取り組み (大阪大学 蛋白研セミナー 2015 口頭発表)

(招待講演) タンパク質の脂質認識機構と脂溶性化合物を扱うための技術開発 (京都府立大学生命環境学部生命分子化学科 第27回鴨叡会・生命分子化学科セミナー 2015 口頭発表)

(Invited lecture) Growth of protein crystals in high-strength hydrogels (3rd International Picobiology Institute Symposium, Center for Advanced Science and Technology (CAST) 2015 口頭発表)

(招待講演) ゲルを利用したタンパク質結晶化法 (第52回日本生物物理学会年会シンポジウム 2014 口頭発表)

(招待講演) 固相ハイドロゲル中でのタンパク質の結晶化 (日本物理学会第69回年次大会シンポジウム 2014 口頭発表)

(招待講演) タンパク質の結晶構造解析から何がわかるか? (神戸薬科大学セミナー 2014 口頭発表)

(Invited lecture) A novel approach for protein crystallization with high-strength hydrogels (The collaborative conference on crystal growth 2014 (3CG 2014) 2014 口頭発表)

(Invited lecture) Hydrogel protects protein crystals from osmotic shock (15th International Conference on the Crystallization of Biological Macromolecules (ICCBM15) 2014 口頭発表)

(招待講演) 固相ゲル結晶化法 ((独)日本学術振興会、回折構造生物第169委員会、第43回研究会 2013 口頭発表)

(招待講演) 蛋白質の固相ゲル結晶育成技術の開発とその機能解析 (第42回結晶成長国内会議(NCCG-42) 2012 口頭発表)

(招待講演) ヒト心臓由来脂肪酸結合タンパク質の脂肪酸認識機構 (NECソフト株式会社VALWAYテクノロジーセンターセミナー 2012 口頭発表)

(Invited lecture) Growth of protein crystals in semi-solid hydrogel (Institute for protein research seminar, Osaka University 2012 口頭発表)

(Invited lecture) Protein Crystallization Controlled by Femtosecond Laser-Induced Cavitation Bubbles (The 12th International Symposium on Laser Precison Microfabrication 2011 口頭発表)

(Invited lecture) Crystal structure of spermidine acetyltransferase from Escherichia coli (2010 INTERNATIONAL POLYAMINE CONFERENCE 2010 口頭発表)

(Invited lecture) Protein Crystallization by Femtosecond Laser Irradiation and Solution Stirring (The 4th Asian Conference on Crystal Growth and Crystal Technology (CGCT-4) 2008 口頭発表)

(招待講演) Crystal Structure analysis of Prostaglandin F2alpha synthase (第30回日本分子生物学会年会・第80回日本生化学会大会合同大会(BMB2007) 2007 口頭発表)

(招待講演) バイオベンチャーと宇宙ビジネス (起業家グループ「Kyoto eggs」セミナー 2006 口頭発表)

(Invited lecture) Protein Crystallization Conditions Database, CrystalT.B. -A Farewell to Random Screening- (American Crystallographic Association (ACA) 2005 口頭発表)

■ 研究業績(特許)
重原子化化合物、膜タンパク質の結晶化を促進するための組成物、および該組成物を用いた膜タンパク質の構造解析方法
 PCT/JP2017/007540

目的物質移行方法、結晶製造方法、組成物製造方法、目的物質移行装置
 WO2012/099180

タンパク質結晶の観測方法
 WO2012/039377

有機高分子結晶製造装置
 特許公開2011-116591

結晶製造方法、凍結結晶製造方法、結晶構造解析方法、結晶化スクリーニング方法、結晶化スクリーニング装置
 PCT/JP2009/050592

ジペプチジルペプチダーゼIVの立体構造
 WO2004/011640

■ 研究業績(その他の活動)
日本結晶学会平成29年度年会 シンポジウム「放射光X線利用による核酸構造研究の新展開」オーガナイザー(2017年)

第16回日本蛋白質科学会 ワークショップ「脂質-タンパク質の相互作用研究の新展開」オーガナイザー(2016年)

第38回日本分子生物学会年会・第88回日本生化学会大会合同大会BMB2015 シンポジウム「脂質構造生物学の新機軸」オーガナイザー(2015年)

第53回日本生物物理学会年会 シンポジウム「脂質活性構造研究の最前線」オーガナイザー(2015年)

日本薬学会第135年会 シンポジウム「脂質活性構造研究の最前線」オーガナイザー(2015年)


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