■ 氏名
枝重 圭祐 (エダシゲ ケイスケ)
EDASHIGE Keisuke

■ 所属部局/職名
Research and Education Faculty, Multidisciplinary Science Cluster, Life and Environmental Medicine Science Unit/Professor
教育研究部 総合科学系 生命環境医学部門/教授

■ 学部・学科・コース等
Faculty of Agriculture and Marine Science, Department of Agricultural Chemistry
農林海洋科学部 農芸化学科

■ 連絡先
住 所
電 話088-864-5195
F A X
E-Mailkeisuke●cc.kochi-u.ac.jp    (送信の際は“●”を半角の“@”に変更して送信してください。)
U R L

■ 出身大学院・研究科等

■ 出身学校・専攻等

■ 取得学位
博士(農学) (論文) 京都大学 応用動物科学
博士(医学) (論文) 高知医科大学 病態医化学

■ 研究職歴

■ 委員歴・役員歴

■ 受賞学術賞

■ 所属学会
日本生化学会
 ( 国内)
日本畜産学会
 ( 国内)
日本実験動物学会
 ( 国内)
基礎生殖免疫学会
 ( 国内)
日本繁殖生物学会
 ( 国内)
The Society for the Study of Reproduction
 ( 国外)
The Society for Cryobiology
 ( 国外)

■ 専門分野
動物生殖工学、低温生物学

■ 主な研究テーマ・活動
動物の生殖細胞の凍結保存後の生存性に関わるタンパク質の機能に関する研究。
凍結保存が難しい生殖細胞の凍結保存技術の開発

■ 研究についてのPR
細胞の凍結保存が成功してから半世紀が立ったが、卵子や胚が凍結保存できる動物種は限られている。絶滅に瀕する哺乳動物種の保護や増殖、魚類のような哺乳動物以外の動物の遺伝資源の保護、移植用の組織や臓器の保存など、今後の細胞の凍結保存技術の改良や開発が期待されている分野も多い。細胞生物学的観点に立ち、遺伝子工学的手法も用いながら、細胞の耐凍性を積極的に向上させる方法によって、従来、凍結保存が困難な細胞や組織の凍結保存を可能にしてゆきたいと考えている。

■ 社会人・生徒を対象として可能な講演・授業(例えば講演題目)
社会人:哺乳動物受精卵の凍結保存の利用
高校生:凍った後に生きている細胞、死んでいる細胞
小・中学生:凍った後に生きている細胞、死んでいる細胞

■ 研究業績(論文・解説)
Equilibrium vitrification of mouse embryos
 Biology of Reproduction (Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai) 82/444, 450 2010 (学術雑誌)

The mechanism by which mouse spermatozoa are injured during freezing
 The Journal of Reproduction and Development (Bo Jin B, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr, Magosaburo Kasai, Keisuke Edashige) 54/4, 265-269 2008 (学術雑誌)

Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival
 Cryobiology (Bo Jin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr, Keisuke Edashige, Magosaburo Kasa) 56/3, 233-240 2008 (学術雑誌)

Development of a reliable in vitro maturation system for zebrafish oocytes
 Reproduction (Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige) 135/3, 285-292 2008 (学術雑誌)

The role of aquaporin3 in the movement of water and cryoprotectants in mouse morulae
 Biology of Reproduction (Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai) 77/2, 365-375 2007 (学術雑誌)

Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes
 Cryobiology (Yohei Yamaji, Delgado M. Valdez Jr., Shinsuke Seki, Ken-ichi Yazawa, Magosaburo Kasai, Chika Urakawa, Bo Jin, F.W. Kleinhans, Keisuke Edashige) 53/2, 258-267 2006 (学術雑誌)

Expression of aquaporin-3 improves the permeability to water and cryoprotectants of immature oocytes in the medaka (Oryzias latipes).
 Cryobiology (Delgado M. Valdez Jr., Takao Hara, Akira Miyamoto, Shinsuke Seki, Magosaburo Kasai, Bo Jin, Keisuke Edashige) 53/2, 160-168 2006 (学術雑誌)

Japanese flounder (Paralichthys olivaceus) embryos are difficult to be cryopreserved by vitrification
 Cryobiology (Keisuke Edashige, Delgado M. Valdez Jr., Takao Hara, Naoya Saida, Shinsuke Seki, Magosaburo Kasai) 53/1, 96-106 2006 (学術雑誌)

Channel-dependent permeation of water and glycerol in mouse morulae
 Biology of Reproduction (Keisuke Edashige, Mitsunobu Tanaka, Natsuko Ichimaru, Satoshi Ota, Ken-ichi Yazawa, Yuko Higashino, Megumi Sakamoto, Yohei Yamaji, Tatsunaga Kuwano, Delgado M. Valdez Jr., F.W. Kleinhans, Magosaburo Kasai) 74/4, 625-632 2006 (学術雑誌)

Water- and cryoprotectant-permeability of mature and immature oocytes in the medaka (Oryzias latipes)
 Cryobiology (Delgado M. Valdez Jr., Akira Miyamoto, Takao Hara, Shinsuke Seki, Magosaburo Kasai, Keisuke Edashige) 50/1, 93-102 2005 (学術雑誌)

Artificial expression of aquaporin-3 improves the survival of mouse oocytes after cryopreservation.
 Biology of Reproduction (Keisuke Edashige, Yohei Yamaji, Magosaburo Kasai) 68/1, 87-94 2003 (学術雑誌)

Morphological appearance of the cryopreserved mouse blastocyste as a tool to identify the type of cryoinjure.
 Human Reproduction 17 (M. Kasai, K. Ito, and K. Edashige. ) /, 1863-1874 2002 (学術雑誌)

Expression of mRNAs of the aquaporin family in mouse oocytes and embryos
 Cryobiology (Keisuke Edashige, Megumi Sakamoto, Magosaburo Kasai) 40/2, 171-175 2000 (学術雑誌)

The expression of mRNAs of the aquaporin family in mouse oocytes and embryos
 Cryobiology 40 (K. Edashige, M. Sakamoto, and M. Kasai) /, 171-175 2000 (学術雑誌)

Factors affecting the survival of frozen-thawed mouse spermatozoa.
 Cryobiology 40 (T.-Z. An, M. Iwakiri, K. Edashige, T. Sakuari, and M. Kasai. ) /, 237-249 2000 (学術雑誌)

Restoration of Resistance to Osmotic Swelling of Vitrified Mouse Embryos by Short-Term Culture
 Cryobiology (Keisuke Edashige, Atsushi Asano, Tie-Zhu An, Magosaburo Kasai) 38/4, 273-280 1999 (学術雑誌)

Viable spermatozoa can be recovered from refrigerated mice up to 7 days after death.
 Cryobiology 38 (T.-Z. An, S. Wada, K. Edashige, T. Sakurai, and M. Kasai. ) /, 27-34 1999 (学術雑誌)

Freeze preservation of spermatozoa recovered from male mice that had been refrigerated after death.
 Journal of Reproduction and Development 45 (T.-Z. An, K. Ikegami, K. Edashige, T. Sakurai, and M. Kasai. ) /, 191-196 1999 (学術雑誌)

Effect of hypotonic stress on the Survival of mouse oocytes and embryos at various stages.
 Cryobiology (Prudencio B. Pedro, Shi-En Zhu, Nobuko Makino, Takashi Sakurai, Keisuke Edashige, Magosaburo Kasai) 35/2, 150-158 1997 (学術雑誌)

Fracture damage of embryos and its prevention during vitrification and warming
 Cryobiology (Magosaburo Kasai, Shi-En Zhu, Prudencio B. Pedro, Ken Nakamura, Takashi Sakurai, Keisuke Edashige) 33/4, 459-464 1996 (学術雑誌)

Cryopreservation of zona-hatched mouse blastocysts
 Journal of Reproduction and Fertility (Shi-En Zhu, Takashi Sakurai, Keisuke Edashige, Takahiko Machida, Magosaburo Kasai) 107/1, 37-42 1996 (学術雑誌)

Reversible priming and protein-tyrosyl phosphorylation in human peripheral neutrophils under hypotonic conditions
 Archives of Biochemistry and Biophysics (Keisuke Edashige, Yoshiya Watanabe, Eisuke F. Sato, Yoshiki Takehara, Kozo Utsumi) 302/2, 343-347 1993 (学術雑誌)

Differentiation of HL-60 cells by phorbol ester is correlated with up-regulation of protein kinase C-α
 Archives of Biochemistry and Biophysics (Keisuke Edashige, Eisuke F. Sato, Kunihiro Akimaru, Magosaburo Kasai, Kozo Utsumi) 299/2, 200-205 1992 (学術雑誌)

Requirement of protein association with membranes for phosphorylation by protein kinase C
 Archives of Biochemistry and Biophysics (Keisuke Edashige, Eisuke F. Sato, Kunihiro Akimaru, Magosaburo Kasai, Kozo Utsumi) 296/1, 296-301 1992 (学術雑誌)

Inhibition of 12-0-tetradecanoyl phorbol B-acetate promoted tumorigenesis by cepharanthine, a biscoclaurine alkaloid, in velation to the in hibitory effect on protein kinase C
 Biochemical Pharmacology (Keisuke Edashige, Toshihiko Utsumi, Kozo Utsumi) 41/1, 71-78 1991 (学術雑誌)

Nonstevoidal antiestrogen suppresses protein kinase C-its inhibitory effect on interaction of substrate protein with membranes
 Cell Structure and Function (Keisuke Edashige, Eisuke F. Sato, Kunihiro Akimaru, Tamotsu Yoshioka, Kozo Utsumi) 16/3, 273-281 1991 (学術雑誌)

■ 研究業績(著書)

■ 研究業績(口頭・ポスター)

■ 研究業績(特許)

■ 研究業績(その他の活動)


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